Download e-book for kindle: Flow Cytometry for Biotechnology by Larry A. Sklar

By Larry A. Sklar

movement cytometry is a delicate and quantitative platform for the size of particle fluorescence. In move cytometry, the debris in a pattern stream in unmarried dossier via a targeted laser beam at premiums of thousands to hundreds of thousands of debris in step with moment. in the course of the time each one particle is within the laser beam, at the order of ten microseconds, a number of fluorescent dyes linked to that particle are excited. The fluorescence emitted from every one particle is accumulated via a microscope goal, spectrally filtered, and detected with photomultiplier tubes.

circulation cytometry is uniquely in a position to the correct and quantitative molecular research of genomic series info, interactions among purified biomolecules and mobile functionality. mixed with computerized pattern dealing with for elevated pattern throughput, those positive factors make move cytometry a flexible platform with purposes at many phases of drug discovery. often, the debris studied are cells, particularly blood cells; move cytometry is used widely in immunology.

This quantity exhibits how stream cytometry is built-in into glossy biotechnology, facing problems with throughput, content material, sensitivity, and excessive throughput informatics with functions in genomics, proteomics and protein-protein interactions, drug discovery, vaccine improvement, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

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Flow Cytometry for Biotechnology by Larry A. Sklar

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